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Figure 1 | Molecular Cancer

Figure 1

From: Vascular endothelial growth factor regulates myeloid cell leukemia-1 expression through neuropilin-1-dependent activation of c-MET signaling in human prostate cancer cells

Figure 1

Mcl-1 is a survival factor in human PCa cells. (a) IHC staining of Mcl-1 in human PCa tissue microarray consisting of normal adjacent tissues, primary PCa and bone metastases. (b) Intracardiac (i.c) injection of ARCaPM cells in athymic mice resulted in metastases to bone and soft tissues. The ARCaPM-C2 subclone was derived from metastatic bone tissues after two rounds of intracardiac inoculation of ARCaPM cells. (c) Endogenous Mcl-1 expression in the ARCaPM model was examined by RT-PCR and western blotting analyses. (d) Effect of Mcl-1 siRNA on ARCaPM cell viability. Subconfluent ARCaPM cells on a 6-well plate were transiently transfected with Mcl-1 siRNA (30 nM) for 72 h. Endogenous expression of Mcl-1 at the mRNA and protein levels, and cell viability of ARCaPM cells as counted with trypan blue staining, were significantly inhibited by Mcl-1 siRNA treatment compared to the control. (e) Effects of Mcl-1 siRNA on ARCaPM cell apoptosis. Subconfluent ARCaPM cells were transfected with Mcl-1 siRNA or control siRNA for 72 h, expression of annexin V was measured by FACS.

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Molecular Cancer

ISSN: 1476-4598