Figure 7

Src kinase-Stat3 signaling mediates VEGF ₁₆₅ induction of Mcl-1 expression in ARCaP _M cells. (a) Effects of NRP1 inhibition on phosphorylation of Src kinases and Stat3. ARCaP_M cells were transfected with NRP1 siRNA or control siRNA for 72 h. (b) Effects of VEGF₁₆₅ on the phosphorylation of Src kinases and Stat3. ARCaP_M cells were treated with VEGF₁₆₅ (10 ng/ml) for the indicated time, and immunoblotting was performed on total lysates (left) and nuclear extracts and cytoplasmic proteins (right). (c) Effects of Src kinases on VEGF₁₆₅ regulation of Mcl-1. ARCaP_M cells were treated with PP2 (10 μM) or DMSO for 2 h before treatment with VEGF₁₆₅ or PBS. (d) Effects of Stat3 depletion on VEGF₁₆₅ induction of Mcl-1. ARCaP_M cells were transfected with Stat3 siRNA or control siRNA (80 nM) for 48 h, serum-starved overnight, then treated with VEGF₁₆₅ (10 ng/ml) or PBS for 2 h.