Figure 2

Constitutive activation of NF-κB and STAT3 in iMyc^Eμ mice occurs early. (A and B) EMSA showing elevated DNA-binding activity of NF-κB (A) and STAT3 (B), respectively, in both premalignant (average spleen weight 80-150 mg) and malignant (average spleen weight ~750 mg) splenic B220-positive B cells from iMyc^Eμ and control BL6 (average spleen weight 80-100 mg) mice. (C) Western blot showing the level of Myc protein in control, premalignant and malignant splenic B cells, defined as above, and in iMyc^Eμ-1 cells. β-actin was used as a loading control. (D and E) Cytokine array (Left panel) and ELISA (Right panel) for IL6 and IL10, showing the levels of IL6 and IL10 protein in splenic B cells (D) and in splenic B220-negative cells (E) from 2-month old control or iMyc^Eμ mice. ImageQuant software was used to quantitate the results of cytokine array, and data are expressed as arbitrary densitometric units (AU) after normalization to positive controls (Pos). "Neg" denotes negative controls.