Figure 3
From: NF-κB/STAT3/PI3K signaling crosstalk in iMycEμ B lymphoma
NF-κB inhibition suppresses growth, causes apoptosis and downregulates STAT3 and Myc activity in iMycEμ-1 cells. (A) MTS/PMS cell proliferation assay, after culture with the NF-kB inhibitor lactacystin (LC) at various concentrations as indicated. Data were normalized to vehicle control, and error bars represent the standard deviation from a representative experiment performed in triplicate. (B) Agarose gel showing DNA fragmentation in sample treated with LC, but not in PBS control. (C) EMSA showing reduced NF-κB DNA-binding after LC treatment. (D) Stabilization of IκB protein after treatment with LC, as determined by Western blotting. (E) Reduced STAT3 DNA-binding activity after NF-κB inhibition, as observed by EMSA. (F) EMSA showing that Myc DNA-binding activity is reduced after LC treatment. (G) Western blot showing that Myc protein levels are reduced after NF-κB inhibition. β-actin was used as a loading control for Western blots. All LC incubations were for 24 hours.