Skip to main content
Figure 8 | Molecular Cancer

Figure 8

From: Prostate apoptosis response protein 4 sensitizes human colon cancer cells to chemotherapeutic 5-FU through mediation of an NFκB and microRNA network

Figure 8

Alterations of microRNA expression following Par-4 overexpression in HT29 cells. (A) Principal component analysis (PCA) of colon cancer cells based on global microRNA expression. Three-dimensional PCA demonstrated that global microRNA expression patterns segregated (with 69.9% of the variance) in accordance with Par-4- (indicated in red) or empty vector-transfected (Vector) HT29 cells (indicated in blue). (B) Unsupervised hierarchical clustering of significantly up- and down-regulated microRNAs (as determined by t-test with 10% FDR) in Par-4- versus empty vector-transfected cells. (C) Validation of microRNA profiles in response to Par-4 overexpression. MicroRNAs identified as altered following Par-4 overexpression were validated by qRT-PCR. Fold changes for miR-18a, miR-193b, mi-195, miR-221, miR-222, miR-30d, miR-30d, miR-34a and miR-7 are indicated. Results are the mean ± S.D. of 4-5 independent experiments. *,** Significantly different between Par-4- and empty vector-transfected cells (P < 0.05).

Back to article page

Molecular Cancer

ISSN: 1476-4598