Figure 10

Differential efficacy of PARP cleavage by quercetin and withaferin A in K562 and K562/Adr cells. A) K562 and K562/Adr cells were either left untreated or treated with different doses of quercetin or withaferin A for 24 h. Cell lysates were analyzed for PARP cleavage by Western analysis. B) K562 and K562/Adr cells were either left untreated (1), 24 h treated with 100 μM of quercetin or 10 μM withaferin A (2), 24 h treated with 100 μM quercetin or 10 μM withaferin A following 2 h prior WA preincubation with 1 mM DTT (3), or 24 h treatment with 1 mM DTT (4). Corresponding cell lysates were analyzed for PARP cleavage by Western analysis.