OTUB1 expression in prostate cancer. (A) Immunohistochemical analysis of OTUB1 expression in prostate cancer. I, prostate sample showing a gland with normal architecture (white arrow head) and a tumor area with a Gleason score of 6 (black arrow head). In the normal gland high OTUB1 expression is observed in basal epithelial cells and in adjacent stromal cells, and increased expression is observed in tumor epithelial cells compared to normal luminal epithelium. II, negative control staining for A. III, example of a prostate tumor with Gleason score of 8 exhibiting moderate staining of epithelial tumor cells. IV, a tumor with Gleason score of 9 showing strong staining of tumor cells. (B) Distribution of OTUB1 expression across prostate tissue samples with different Gleason grades are shown as bar graphs while relative numbers are shown as pie charts. Chi-square p value comparing OTUB1 expression in malignant versus non-malignant prostate tissues and OTUB1 expression across the different histological Gleason score grades are shown. OTUB1 IR is independent of Gleason score; Chi-square p value across Gleason score grades is 0.7.