OTUB1 de-ubiquitinating enzyme promotes prostate cancer cell invasion in vitro and tumorigenesis in vivo

Iglesias-Gato, Diego; Chuan, Yin-Choy; Jiang, Ning; Svensson, Charlotte; Bao, Jing; Paul, Indranil; Egevad, Lars; Kessler, Benedikt M; Wikström, Pernilla; Niu, Yuanjie; Flores-Morales, Amilcar

doi:10.1186/s12943-014-0280-2

Molecular Cancer

Table 1 Proteins regulated upon OTUB1 knockdown in LNCaP-FGC cells

From: OTUB1 de-ubiquitinating enzyme promotes prostate cancer cell invasion in vitro and tumorigenesis in vivo

Gene name	Uniprot	Log2(siOTUB1_1/siControl)	Log2(siOTUB1_2/siControl)	Mean (SD)
ACPP	P15309	−7.23	−3.08	−5.15 (2.94)
ACTA2	P62736	−3.52	−5.65	−4.58 (1.51)
SHROOM3	Q8TF72	−3.75	−3.24	−3.49 (0.36)
KLK3	P07288	−3.52	−2.72	−3.12 (0.57)
TAGLN	Q01995	−2.44	−3.36	−2.9 (0.65)
DES	P17661	−3.69	−1.95	−2.82 (1.23)
KPRP	Q5T749	−2.33	−2.75	−2.54 (0.3)
ST14	Q9Y5Y6	−1.03	−3.95	−2.49 (2.07)
DCD	P81605	−2.60	−2.34	−2.47 (0.19)
CASP14	P31944	−3.23	−1.44	−2.34 (1.27)
STAM	Q92783	−1.36	−2.18	−1.77 (0.58)
ACTC1	P68032	−1.05	−2.36	−1.7 (0.93)
ANXA2	P07355	−1.17	−2.17	−1.67 (0.71)
HSPB1	P04792	−1.20	−1.91	−1.56 (0.5)
DTNB	O60941	−1.57	−1.55	−1.56 (0.02)
SNX2	O60749	−0.82	−1.84	−1.33 (0.72)
BLMH	Q13867	−1.44	−1.09	−1.27 (0.25)
MICAL1	Q8TDZ2	−1.02	−1.43	−1.23 (0.29)
WDR7	Q9Y4E6	−1.17	−1.26	−1.21 (0.06)
MALT1	Q9UDY8	−1.28	−0.99	−1.13 (0.2)
RAB8A	P61006	−0.75	−1.23	−0.99 (0.34)
NOC2L	Q9Y3T9	−1.04	−0.89	−0.96 (0.1)
ARHGAP35	Q9NRY4	−1.19	−0.73	−0.96 (0.32)
UBE2K	P61086	−1.09	−0.81	−0.95 (0.2)
USP39	Q53GS9	−1.19	−0.69	−0.94 (0.36)
HMGCS2	P54868	−0.72	−1.12	−0.92 (0.28)
QSOX1	O00391	−0.92	−0.89	−0.9 (0.02)
TOR1AIP1	Q5JTV8	−0.75	−0.91	−0.83 (0.11)
ADAM9	Q13443	−0.86	−0.73	−0.79 (0.09)
LAMC1	P11047	−0.77	−0.80	−0.79 (0.02)
NUP88	Q99567	−0.71	−0.86	−0.79 (0.1)
YWHAB	P31946	−0.68	−0.73	−0.71 (0.03)
NDRG1	Q92597	−0.65	−0.75	−0.7 (0.07)
FAM160B1	Q5W0V3	−0.66	−0.66	−0.66 (0)
DBR1	Q9UK59	0.65	0.59	0.62 (0.05)
SLC4A2	P04920	0.89	0.75	0.82 (0.1)
POLA1	P09884	0.69	1.14	0.91 (0.32)
DDX42	Q86XP3	0.92	1.13	1.02 (0.15)
TTN	Q8WZ42	1.50	1.22	1.36 (0.2)
PTGFRN	Q9P2B2	2.04	1.66	1.85 (0.27)

Proteins showing changes in expression after OTUB1 knockdown using two different siRNA, compared to control siRNA transfected LNCaP-FGC cells. Androgen regulated proteins are highlighted. Fold changes are shown as log2 of the ratio between the intensity of the proteins in the siRNA transfected and control siRNA transfected cells. Mean values and Standard deviation (SD) for both experiments are shown on the right column.

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