TIMP-1/MMP2,9 singaling showed obvious change when parental C4-2 cells cultured in the CM from THP-1 siTR/C4-2 siTR4.
A: mRNA level of qPC Rarray; B: Protein level of TIMP-1/MMP2/MMP9 signaling. * Parental C4-2 cells were treated for 24 hours with the CMs from the co-culture of PCascr/THP-1 scr (control) and C4-2 siTR4/THP-1 siTR4 (study). mRNA was isolated and then metastasis-related genes qPCR array were applied to screen for the potential factors in knocked-down of TR4-reduced macrophage recruitment-PCa cells invasion. These folds represent the ratio of study/control. # Western blotting to confirm the change of TIMP-1/MMP2/MMP9 signaling. Left line is control group, right line is study group.