Figure 2

Loss of host derived MMP13 leads to decreased tumor metastasis to the liver. (a) Representative liver cross sections stained with Haematoxylin & Eosin of wildtype and MMP13−/− mice with normal or steatotic livers show an increased tumor burden in wildtype mice compared to MMP13−/− mice (n = 5). Tumors are denoted by black dashed line. Quantification of metastatic tumor burden by (b) liver weight, (c) tumor burden measured as percent liver weight to total body weight, metastatic seeding by quantification of (d) tumor number and (e) tumor area per liver section in normal and steatotic livers. Statistical analysis was performed using GraphPad Prism software. Data was analyzed by using one-way ANOVA followed by Newman-Keuls Multiple Comparison Test. P values are represented by stars where: * ≤ .05, ** ≤ .01, and *** ≤ .001.