Figure 4

Effect of knockdown of MMP13 in tumor cells on various hallmarks of cancer in vitro . Immunohistochemical staining for MMP13 in representative sections of (a) murine MC38 tumor in the mouse liver and (b) human colorectal cancer metastasis to the liver at 20X magnification. Scalebar represents 100 microns. Inset shows individual tumor cells at 63X magnification. MTT assay shows no change in proliferation after loss of MMP13 in vitro in (c) MC38 or (f) HCT116; control and MMP13 knockdown cell lines. Knockdown of MMP13 leads to decreased ability of tumor cells to migrate in vitro as determined by a transwell migration assay in (d) MC38 and (g) HCT116 cell lines. Knockdown of MMP13 leads to decreased ability of tumor cells to invade in vitro as determined by the transwell invasion assay in (e) MC38 and (h) HCT116 control and knockdown cell lines. Statistical analysis was performed using GraphPad Prism software. Data was analyzed by using one-way ANOVA followed by Newman-Keuls Multiple Comparison Test. P values are represented by stars where: *** P ≤ 0.0001 when compared to the respective non-silencing control treated cell lines.