The extent of KIT expression
delays myeloid differentiation in cells with functional RUNX1. (A) CD117 cytofluorimetric analysis showing ectopic KIT expression in 32DKIT-Low and 32DKIT-High stable clones. (B) Growth curves showing increased proliferation of 32DKIT-Low and 32DKIT-High clones relative to the 32DEV control clone in response to different concentrations of G-CSF. (C-D) CD11b cytofluorimetric analysis shows that increasing KIT expression delays G-CSF-induced differentiation in a G-CSF dose-dependent (C) and time-dependent manner (D). See text and Additional file 4: Table S3 for statistical significance.