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Figure 5 | Molecular Cancer

Figure 5

From: Receptor protein tyrosine phosphatase beta/zeta is a functional binding partner for vascular endothelial growth factor

Figure 5

VEGF directly interacts with RPTPβ/ζ in a VEGFR-independent manner. (A) Serum-starved untreated or VEGF165-stimulated HUVEC lysates were immunoprecipitated for VEGFR2 and analyzed by Western blot for the presence of RPTPβ/ζ or VEGFR2 (up). No direct interaction between VEGFR2 with RPTPβ/ζ was observed by performing in situ PLA in HUVEC (down). The RPTPβ/ζ-ανβ3 interaction was used as a positive control. Data are from two independent experiments. (B) Formation of VEGF-RPTPβ/ζ complexes as evidenced by in situ PLA in HUVEC, untreated or after addition of exogenous VEGF165 (10 ng/ml) at 24 h. The VEGF-VEGFR2 interaction was used as a positive control. Data are from five independent experiments. (C) Formation of VEGF-RPTPβ/ζ complexes as evidenced by in situ PLA in HUVEC in the absence or the presence of bevacizumab (250 μg/ml). The box plots in B and C indicate the median, mean and range of the detected signals (n > 20 image fields with ~4 cells per image per sample type, each sample run at least in duplicate) from three independent experiments. (D) Immunofluorescence images stained for NCL (green) and nucleus (blue) in serum starved HUVEC treated for 5 h at 37°C with VEGF165 (10 ng/ml) in the presence or the absence of bevacizumab (250 μg/ml). Representative pictures from three independent experiments. Scale bars in all cases correspond to 10 μm.

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