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Figure 5 | Molecular Cancer

Figure 5

From: Notch1 signaling regulates the epithelial–mesenchymal transition and invasion of breast cancer in a Slug-dependent manner

Figure 5

Jagged1-mediated activation of Notch signaling induces EMT and cannot rescue the changes caused by Notch1 interference. shNotch1 + Jagged1 group: MCF-7 or MDA-MB-231 cells were treated with Jagged1 for 48 h after stable shNotch1 transfection. (A) Immunofluorescence analysis of E-cadherin and vimentin in MCF-7 cells. DAPI staining was used to detect nuclei and is merged with E-cadherin and vimentin in their respective panels. The cells were observed under an immunofluorescence microscope at 400× magnification. (B) Western blot analysis of the EMT-related markers E-cadherin, occludin, N-cadherin, and vimentin in MCF-7 and MDA-MB-231 cells. (C) The mRNA expression levels of E-cadherin, occludin, N-cadherin, and vimentin were estimated by real-time PCR. (D) Western blot analysis of nuclear β-catenin in MCF-7 and MDA-MB-231 cells. The data are from three independent experiments. Column: mean; bar: SD. The symbol * represents a significant difference (P < 0.05).

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Molecular Cancer

ISSN: 1476-4598