Figure 7

Notch1 regulates Slug expression by enhancing its promoter activity. (A) and (B) The level of Slug expression was evaluated by western blot and real-time PCR after MDA-MB-231 cells were stably transfected with shNC or shNotch1. (C) and (D) The Slug expression level following treatment with Jagged1 ligand for 48 h was estimated by western blot and real-time PCR. (E) The schematic diagram shows the construction of the pGL3-Slug promoter and its negative control pGL3-basic. (F) Luciferase reporter assays were carried out in MDA-MB-231 cells, which were cotransfected with the pGL3-Slug promoter or its negative control pGL3-basic and N1ICD overexpression plasmid pcDNA3.1(+) or its negative control plasmid pcDNA3.1. Each independent experiment was repeated three times. Column: mean; bar: SD. The symbol * represents a significant difference (P < 0.05).