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Figure 5 | Molecular Cancer

Figure 5

From: Inhibition of nucleoporin member Nup214 expression by miR-133b perturbs mitotic timing and leads to cell death

Figure 5

Excess miR-133b enhances chromosomal abnormalities. (a) and (b) HCT116 cells were transiently transfected with empty vector (1 μg), pSB-miR-133b (1 μg), or pSB-miR-133b (1 μg) and pCMV-Myc-CAN/Nup214 (1 μg) and synchronized. Immuno-staining was done with antibody against p-H3 and nuclei were stained with DAPI at 12 and 14 h from second thymidine release. Cells were visualized under a fluorescence microscope. (a) Representative images are shown and the defects indicated by white arrows. Scale bar represents 10 μm. Images represent 60× magnification. (b) Percentages of mitotic abnormalities were calculated and plotted as shown. Data represents three independent experiments and shown as average ± S.D.

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Molecular Cancer

ISSN: 1476-4598