WB analysis to show the expression of in/active GSK3, cyclin D1 and p53 proteins at various stages of OSCC. (A) Representative blot showing the expression of GSK3α, GSK3β, pSer21GSK3α, pSer9GSK3β, cyclin D1 and p53 in various normal, PMLs and OSCC samples. β-Actin was used as a loading control in this experiment. (B) The mean and SD of each protein band has been plotted for the normal, PML and OSCC samples. Statistical analysis was performed using Student’s t-test. Statistical significance was observed among various groups: **p = 0.001, ***p < 0.0001 as indicated in the figure. The comparison of (a) pS9GSK3β, (b) pSer21GSK3α, (c) cyclin D1, and (d) p53 protein expression among the groups of samples. (C) A positive correlation was obtained in different pairs: (a) pSer9GSK3β and cyclin D1 (p = 0.002), (b) pSer21GSK3α and cyclin D1 (p = 0.001), (c) pSer21GSK3α and p53 (p = 0.0001), and (d) pSer9GSK3β and p53 (p = 0.01).