Figure 5

WB analysis to show the expression of in/active GSK3, cyclin D1 and p53 proteins at various stages of OSCC. (A) Representative blot showing the expression of GSK3α, GSK3β, pSer²¹GSK3α, pSer⁹GSK3β, cyclin D1 and p53 in various normal, PMLs and OSCC samples. β-Actin was used as a loading control in this experiment. (B) The mean and SD of each protein band has been plotted for the normal, PML and OSCC samples. Statistical analysis was performed using Student’s t-test. Statistical significance was observed among various groups: **p = 0.001, ***p < 0.0001 as indicated in the figure. The comparison of (a) pS⁹GSK3β, (b) pSer²¹GSK3α, (c) cyclin D1, and (d) p53 protein expression among the groups of samples. (C) A positive correlation was obtained in different pairs: (a) pSer⁹GSK3β and cyclin D1 (p = 0.002), (b) pSer²¹GSK3α and cyclin D1 (p = 0.001), (c) pSer²¹GSK3α and p53 (p = 0.0001), and (d) pSer⁹GSK3β and p53 (p = 0.01).