Figure 3
From: Deregulated microRNAs in triple-negative breast cancer revealed by deep sequencing
RT-PCR validations of the differentially expressed miRNAs in the miR-301b-130b cluster and the miR-497-195 cluster. (A) A conceptual diagram illustrates the genomic loci harboring the 5p miRNA forms (brown) and 3p forms (orange) in the miR-301b-130b cluster. Relative expression levels of (B) miR-301b-5p, (C) miR-130b-5p, and (D) miR-130b-3p in triple-negative breast cancer tissues (n = 19) and adjacent normal tissues (n = 4) are shown. All three miRNAs in the miR-301b-130b cluster were markedly up-regulated in triple-negative breast cancer. (E) A conceptual diagram illustrates the genomic loci harboring the 5p miRNA forms (brown) in the miR-497-195 cluster. Relative expression levels of (F) miR-497-5p and (G) miR-195-5p in 23 samples are shown. Both miRNAs in the miR-497-195 cluster were down-regulated in triple-negative breast cancer. Each bar represents the mean of triplicate measurements ± the SD.