Amino acids 115–125 are required for IRF5 to regulate cell migration. A) Schematic representation of full-length IRF5 highlighting specific protein domains such as the DNA binding domain (DNA BD), nuclear exit signal (NES), nuclear localization signals (NLS) and autoinhibitory domain (AID). Mutants of full-length IRF5 are depicted below; dotted lines show deleted region. B) Expression of FLAG-tagged mutant IRF5 proteins in 231 cells. C) Graphical summary of data from migration assays comparing full-length IRF5 to the illustrated mutants shown in A); statistical analysis is compared to EV control. D) Internal deletion mutants of full-length IRF5 are shown; empty boxes indicate deleted amino acids. E) Expression levels of FLAG-tagged internal deletion mutants of IRF5. F) Similar to C) summarizing data from the migration assay comparing EV migratory function to full-length and mutant IRF5 function in 231 cells; statistical analysis is compared to EV control. Bars denote statistical differences between mutants and full-length IRF5. Data are from at least 3 independent experiments performed in duplicate; *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.0001.