Table 5 Sequences of the primers flanking potential binding sites (BS) of Ik-1 and MZF1 within the human IGF-IR promoter used in the RT-PCR reactions following the ChIP assay

Ik-1 BS2 (Forward)

5′-CGG GGG CAT TGT TTT TGG AG-3′

Ik-1 BS2 (Reverse)

5′-CGG GTT CCC CAA GAC GTG-3′

Ik-1 BS3 ( Forward)

5′-TCT TGT TTA CCA GC ATTA ACT CGC-3′

Ik-1 BS3 (Reverse)

5′-CCT CTC TCG AGT TCG CCT G-3′

Ik-1 BS4 (Forward)

5′-CGC CGC TTT GTG TGT GTC-3′

Ik-1 BS4 (Reverse)

5′-GCC GCC TCC TCC CTC A-3′

MZF1 BS2 (Forward)

5′-GCG GGG GCA TTG TTT TTG GA-3′

MZF1 BS2 (Reverse)

5′-CCG GGT TCC CCA AGA CGT G-3′

MZF1 BS3 (Forward)

5′-GCG CGT GTC TCT GTG TGC-3′

MZF1 BS3 (Reverse)

5′-GCG AGT TAA TGC TGG TAA ACA A-3′

MZF1 BS4 (Forward)

5′-GTG TGT GTC CTG GAT TTG GGA-3′

MZF1 BS4 (Reverse)

5′-GCA GAA ACG CGG AGT CAA AAT-3′

MZF1 BS5 (Forward)

5′-CGG CCC TTC GGA GTA TTG T-3′

MZF1 BS5 (Reverse)

5′-CAA GTC TCA AAC TCA GTC TTC G-3′