Figure 6

Methylation alterations associated with BRAF ^V600E driver mutations in primary tumor samples. A) Density plots of the median methylation intensity of each CpG site in BRAF ^V600E samples and BRAF ^WT samples. Methylation loci with Δβ > 0.1 were labeled with black dots. The plot indicates a global DNA methylation loss associated with BRAF^V600. B) Heat-map showing differential methylation signals between BRAF ^V600E and BRAF ^WT samples, indicating a dormant methylation loss in BRAF ^V600E samples. C) Boxplots showing significantly lower expressions of DNMT3A in TCGA BRAF ^V600E versus BRAF ^WT samples. D) Boxplot of DNMT3A expression level on A375 melanoma cells before and after the treatment of vemurafenib.