Figure 2
From: Inhibition of MDM2 homodimerization by XIAP IRES stabilizes MDM2, influencing cancer cell survival
Determination of XIAP IRES RNA binding to MDM2 C-terminal RING domain protein. A, excitation and emission spectra of the MDM2 RING domain protein. B, fluorescence intensity (FI) calculation of MDM2 RING domain protein in the presence of increasing concentrations of XIAP IRES RNA and control RNAs (XIAP non-IRES, XIAP IRES mutation and MYCN IRES), relative to the FI in the absence of RNA, to derive the Kd. C, thermodynamic measurement of the binding of XIAP IRES RNA (left) and XIAP non-IRES (right) to MDM2 RING protein using isothermal titration calorimetry (ITC). The upper box is the raw heating power over time and the lower box is a fit of the integrated energy values, normalized for each injection. We performed each ITC experiment three times or more.