Figure 2

SapC-DOPS induces apoptosis in neuroblastoma cell lines. A) Viability of SK-N-SH, IMR-32 and human Schwann cells measured by the MTT assay. **represents the lowest concentration from which P value is significant i.e., < 0.001. B) Microscopic images of SK-N-SH cells after treatment with 350 μM DOPS or 50 μM SapC-DOPS. Arrows indicate morphological changes such as cell shrinkage and chromatin condensation. Original magnification: 20x). C) Changes in cell cycle following treatment of SK-N-SH cells with 50 μM SapC-DOPS for 24 h. The value represents percentage of cells in Sub-G1 region. *P < 0.05, **P < 0.001 when compared to control. Points, mean of three to five independent experiments; bars, SE.