Figure 2
From: MicroRNA-31 controls G protein alpha-13 (GNA13) expression and cell invasion in breast cancer cells
GNA13 protein expression correlates with aggressiveness of breast cancer cells. (A) Enforced expression of GNA13 in MCF-10a cells enhances both basal and FCS-induced invasion. Relative invasion is reported as fold change relative to pMSCV-Control (−FCS). The inset shows immunoblot analysis of GNA13 and tubulin in the same cells. (B) Knockdown of GNA13 protein expression inhibits basal and FCS-induced invasion of MDA-MB-231 cells. The inset shows the knockdown efficiency of GNA13. Invasion is reported as the relative fold change in relation to MDA-MB-231-Sh-Scr (−FCS). Panels (C) and (D) - cell proliferation data of MCF-10a and MDA-MB-231 cells, respectively. MTT assays were performed as described in methods and absorbance at 490 is plotted in the y-axis and hours on the x-axis as shown (*, p < 0.05).