Figure 2
From: MiR-377 targets E2F3 and alters the NF-kB signaling pathway through MAP3K7 in malignant melanoma
MiR-377 effect on melanoma cells proliferation. (A) MiR-377 expression in normal melanocytes (NHEM) or mel33B1 (B1) or mel-14PA (14) melanoma cells after stable transfection with HTR control vector (B1 HTR, 14 HTR) or vector expressing miR-377 (B1 377, 14 377). Rnu48 served as normalizing control. (B) The growth of mel-14PA (left) and mel33B1 (right) melanoma cell lines stably transfected with HTR control vector or vector expressing miR-377 was assessed using the crystal-violet method for 72–96 h. Data is represented as mean ± SEM from three independent experiments. *signifies p < 0.05. (C) The growth of mel-14PA melanoma cell lines stably transfected with HTR control vector or vector expressing miR-377 was assessed using the xCELLigence real-time system. For determination of statistical significance, a repeated measures two-way ANOVA with Bonferroni post-hoc tests was performed; *signifies p < 0.05, ***signifies p < 0.001.