Figure 4

Box plot representation of the expression of FOXM1 regulatory network genes in OAC patient samples. (A-C) Box plots of mRNA levels of the FOXM1 target genes in normal oesophageal (left panels) and oesophageal adenocarcinoma (OAC) tissue samples (right and middle panels). Where indicated, the OAC samples are further partitioned according to high (right panel; n = 31) or low (middle panel; n = 27) FOXM1 expression. High FOXM1 expression was defined as mRNA levels that were greater than two standard deviations and greater than two-fold of the mean FOXM1 level in the normal tissues. Genes are grouped according to representing FOXM1 targets shared between OE33 and U2OS cells (A), specifically bound in OE33 cells (B) or encoding components of the MMB and DREAM complexes (C). The mRNA level relative to the median level of the normal tissues (taken as 1) is shown. Boxes represent the interquartile range and the median value is indicated by the horizontal line. Open black circles represent outliers. The dotted line in (A) is the average median expression value of all the genes in the particular sub-panel (value indicated in red). Statistical significance of the change in expression between OAC and normal tissue, normal and low FOXM1 OAC tissue or low FOXM1 and high FOXM1 OAC tissue, is indicated in the rightmost panel of the two panels being compared (** P-value <0.01; * P-value <0.05). (D) Summary of the changes in expression of genes encoding MMB/DREAM complex components in OAC samples (red and blue represent down and up regulated in OAC respectively).