Figure 1

Human glioma stem cell spheres SHG-139S derived from SHG-139 after culture in NSCM. A: SHG-139 at the 20^th generation, light microscope (×400); B, C: SHG-139S, derived from SHG-139 at the 1^st and 10^th generations, light microscopy (×200); D-F: In DMEM/F12 medium without EGF, bFGF and fetal bovine serum, SHG-139s cells were adherent, cell morphology changes at 6 h, 24 h, 48 h, light microscope (×200).