Figure 2

ITGB3 is an miR-320a target gene. (A) Target sequence of miR-320a in ITGB3 3′-UTR predicted by TargetScan, as well as the mutated sequence. (B) Luciferase reporter assays were performed in ACCM and SACC-LM cells co-transfected with miR-320a-mimics and reporter vectors carrying a ITGB3 3′-UTR with a wild-type (ITGB3 3′-UTR) or mutated miR-320a (ITGB3 3′-UTR mut) response element. *P < 0.05 vs. mock transfection. (C) Integrin β3 expression, as well as FAK and Src phosphorylation, was assessed using western blot analysis. β-actin was used as an internal control. (D) Colocalization of integrin β3 and the cytoskeletal protein actin was illustrated by immunofluorescence staining. Nuclei: blue, scale bar: 10 μm.