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Table 2 Serum concentrations of cytokines from untreated, DMBA Only- and DMBA + ROSI-treated strains

From: Opposing roles for mammary epithelial-specific PPARγ signaling and activation during breast tumour progression

  PPARγ-WT PPARγ-MG KO
  Untreated (n = 5) DMBA Only (n = 4) DMBA + ROSI (n = 4) Untreated (n = 5) DMBA Only (n = 4) DMBA + ROSI (n = 4)
Cytokine [signif] mean ± SD; all values expressed as pg / ml
IL-1α 90.6 ± 56.6 93.9 ± 106.3 48.9 ± 32.9 177.0 ± 206.4 39.8 ± 66.1 36.8 ± 46.0
IL-1β 372.6 ± 105.3 3016.0 ± 5279.0 309.0 ± 74.0 195.4 ± 25.9 870.9 ± 1383.0 116.7 ± 91.1
IL-2 62.8 ± 47.0 42.4 ± 27.9 64.6 ± 80.3 34.9 ± 15.0 24.8 ± 6.1 41.8 ± 32.9
IL-3 6.8 ± 15.2 63.4 ± 111.5 16.0 ± 13.6 12.9 ± 6.6 25.4 ± 40.9 6.8 ± 5.6
IL-4 [gg] 16.1 ± 7.9 16.4 ± 3.2 20.1 ± 10.0 11.8 ± 1.5 10.4 ± 1.2 11.0 ± 1.1
IL-5 40.0 ± 16.3 280.7 ± 454.0 23.2 ± 17.2 ND 77.4 ± 154.8 4.6 ± 9.3
IL-6 18.0 ± 16.4 215.5 ± 387.5 20.7 ± 12.0 5.7 ± 1.5 62.7 ± 106.6 14.5 ± 8.5
IL-9 ND ND ND ND ND ND
IL-10 [ggg] 97.3 ± 64.6 163.2 ± 114.2 117.6 ± 48.8 23.5 ± 9.5 39.7 ± 19.9 52.0 ± 20.9
IL-12(p40) [tt] 694.9 ± 299.5 1212.0 ± 302.4 625.3 ± 314.0 554.5 ± 176.1 971.7 ± 306.8 665.1 ± 227.3
IL-12(p70) ND ND ND ND ND ND
IL-13 [g] 654.1 ± 638.6 410.6 ± 437.3 440.6 ± 380.4 95.4 ± 71.7 124.9 ± 33.2 248.0 ± 91.7
IL-17A 32.3 ± 23.8 588.5 ± 1114.0 34.9 ± 19.5 2.1 ± 3.2 150.3 ± 294.5 7.8 ± 12.6
Eotaxin [gg] 1552.0 ± 1593.0 1141.0 ± 890.4 2016.0 ± 743.7 400.4 ± 397.5 620.8 ± 482.6 188.4 ± 163.4
G-CSF 199.6 ± 85.9 1631.0 ± 929.1 3393.0 ± 5274.0 149.1 ± 46.6 564.3 ± 375.5 433.7 ± 121.9
GM-CSF [gggg] 420.2 ± 218.5 384.4 ± 86.8 375.3 ± 94.4 173.0 ± 68.1 * 148.6 ± 47.4 110.1 ± 75.6 Δ
IFN-γ [g] 35.4 ± 38.6 26.6 ± 10.5 23.9 ± 11.3 15.3 ± 6.8 6.8 ± 6.4 9.6 ± 7.0
KC 46.4 ± 62.2 25.3 ± 9.4 41.1 ± 28.2 8.8 ± 1.6 23.1 ± 11.5 14.9 ± 2.4
MCP-1 218.2 ± 132.6 1062.0 ± 1493.0 286.4 ± 156.2 75.8 ± 38.7 190.7 ± 132.6 95.9 ± 17.7
MIP-1α [gg] 67.2 ± 47.2 87.8 ± 38.9 82.5 ± 54.7 45.1 ± 15.2 26.3 ± 8.9 37.1 ± 11.4
MIP-1β 51.9 ± 36.9 525.1 ± 909.0 39.4 ± 13.2 15.2 ± 4.0 185.8 ± 335.4 17.4 ± 15.8
RANTES 31.5 ± 23.1 71.6 ± 76.2 40.7 ± 19.4 37.1 ± 8.5 82.1 ± 97.6 27.2 ± 3.9
TNF-α 1128.0 ± 992.2 19244.0 ± 36869.0 538.0 ± 207.5 272.8 ± 81.9 5763.0 ± 10948.0 363.3 ± 352.8
VEGF 144.6 ± 32.8 (7) 489.7 ± 420.8 (3) ** 132.8 ± 33.7 (4) # 254.4 ± 57.0 (8) 167.5 ± 40.9 (4) # 137.3 ± 22.4 (4)
Leptin 22290.0 ± 11260.0 (7) 8040.0 ± 1968.0 (3) 25820.0 ± 18020.0 (4) 13680.0 ± 1899.9 (8) 32140.0 ± 40310.0 (4) 39560.0 ± 44040.0 (4)
PGE Metabolites 560.1 ± 676.7 (5) 76.7 ± 13.7 (4) 93.9 ± 51.6 (4) 141.3 ± 122.2 (4) 335.0 ± 158.5 (3) 229.0 ± 202.7 (3)
  1. Concentrations reported as mean ± standard deviation (SD) and expressed as pg/ml. Except for VEGF, leptin, and PGE metabolites, which were analyzed with separate ELISA kits, all cytokine concentrations were obtained by a multiplex array. *, significantly different from Untreated PPARγ-WT, p < 0.05; **, significantly different from Untreated PPARγ-WT, p < 0.01; #, significantly different from DMBA Only-treated PPARγ-WT, p < 0.05; Δ, significantly different from DMBA + ROSI-treated PPARγ-WT, p < 0.05. g, genotype different, p < 0.05; gg, genotype different, p < 0.01; ggg, genotype different, p < 0.001; gggg, genotype different, p < 0.0001; tt, treatment different p < 0.01; ND, not detectable.