Figure 2From: Lipid starvation and hypoxia synergistically activate ICAM1 and multiple genes in an Sp1-dependent manner to promote the growth of ovarian cancer Sp1 and HIFs contribute to the synergistic activation of multiple genes in OVSAYO cells under SSH. a) Synergistic activation of genes under SSH. N and H indicate normoxia (20% O2 for 16 h) and hypoxia (1% O2 for 16 h), respectively. Data shown represent the mean (n = 3) ± SD. b) Activation of multiple genes under hypoxia is dependent on HIFs. Western blotting is also shown for HIFs. Data shown are the mean (n = 3) ± SD. c) The ICAM1 promoter region and predicted Sp1 binding sites (ref. 19). PCR-amplicons generated in the reporter gene assay are shown in Additional file 2: Figure S1a. Bold bars: mutated sites (Sp1-mut 1 and 2); underlined nucleotide sequences: regions deleted by mutagenesis. d, e) Luciferase reporter gene assay. Plasmids were co-transfected with the indicated vectors, and luciferase activities were measured at 24 h post-transfection. Luciferase activities measured in cells transfected with the empty vector were defined as 100%. Data shown are the mean (n = 3) ± SD. *P = 0.0018; **P = 0.075.Back to article page