The anti-proliferative effect of Raf265 on HT29 and HCT116 cells. A. Cells were treated with Raf265 at 0–50 μM and MTT assay was performed. B. Cells were suspended in the solidified agarose at the indicated concentrations of Raf265. Representing images under a phase-contrast microscopy at 40× magnification and an amplified view at 400× magnification were shown at the upper panel. The number of colony formed was then counted and the bar chart presenting the average number of colony formed was shown at the lower panel.