PP4C was overexpressed in CRC tissues and cell lines. (A) The PP4C mRNA expression in CRC specimens and paired adjacent non-cancer colorectal tissues was determined by qRT-qPCR. (B) Representative images show PP4C expression in normal tissues adjacent to colorectal tissue (left) and colorectal cancer (right) (Original magnification, 100×). (C) Western blotting analysis of PP4C expression in five CRC cell lines. ** represents p < 0.01.