Skip to main content
Figure 1 | Molecular Cancer

Figure 1

From: Characterization of 46 patient-specific BCR-ABL1 fusions and detection of SNPs upstream and downstream the breakpoints in chronic myeloid leukemia using next generation sequencing

Figure 1

Schematic localization of primers. Forward primers hybridizing in exon 13 of the BCR gene are symbolized by arrows pointing to the right and reverse primers spanning the intron 1 of gene ABL1 are symbolized by arrows pointing to the left. Colors of arrows for each gene illustrate two-rounds of multiplex LR-PCR. LR-PCR products were obtained in 25/48 cases from the first round PCR using 1 forward primer (red color) localized in BCR exon 13 and 10 reverse primers (red color) spanning the whole ABL1 intron 1 [9]. LR-PCR products were obtained in the remaining 23/48 cases from the second round PCR with 1 forward primer (blue color) hybridizing to sequence of BCR exon 13 and 20 reverse primers (blue colors) hybridizing to ABL1 intron 1 [7,10].

Back to article page

Molecular Cancer

ISSN: 1476-4598