Figure 1

Expression of CCDC26 ncRNA. A: Estimated absolute number of CCDC26 mRNA molecules per cell in representative hematopoietic and nonhematopoietic cell lines, shown in the log scale. The absolute number of mRNA molecules per K562 cell of HPRT1, a house keeping gene, and BCR/ABL, an oncogene expressed in K562 cells, was measured as 21+/−5 and 45+/14, respectively. B: A map of human chromosome 8q24, showing the location of the CCDC26 mRNA long and short variants (Genbank accession numbers: NR_130918 and NR_130917, respectively), other minor variants (NR_130919 and NR_130920) containing exon 2A, transcripts including hAK015428, transcriptional hot site 1 (THS1) and THS2, and scores of nuclear and whole cell transcripts in K562 cells. These scores are reprinted from the Human Feb. 2009 (GRCh37/hg19) assembly of the UCSC Genome Browser (http://genome.ucsc.edu/). C: Estimated absolute number of CCDC26, intron THS1, THS2 and hAK015428 transcripts in a K562 cell. D: Quantification of RNA in isolated nuclei and whole cells by quantitative PCR. For each assay, the same amount of RNA was used to synthesize cDNA. The value for HPRT in whole cell RNA was used as a standard. SNORA74, a snoRNA strictly located in nuclei, was used as a control for purity of the nuclear fraction.