Figure 5From: Quercetin inhibits HGF/c-Met signaling and HGF-stimulated melanoma cell migration and invasionOverexpression of PAK or FAK partially reversed the migration-inhibition effect of quercetin. A2058 cells were transiently transfected with a FAK-expressing construct, a PAK-expressing construct, or an empty vector for 24 h. (A) Expression levels of FAK (or PAK) on cells that transfected with FAK (or PAK)-expressing construct. (B) After transfection, cells were treated with quercetin for 24 h, and the effects of quercetin on FAK (or PAK) overexpressed cells were determined by immunoblotting. (C) Cell migratory abilities were measured by migration chamber assay after treated with quercetin for 48 h. The relative quantitative determinations of migrated cells were calculated with 5 fields counted per experiment. The data shown here are the mean ± S.D. from three independent experiments. # P < 0.05, *P < 0.05 and ** < 0.01.Back to article page