Figure 3
Restin overexpression inhibited migration and invasiveness of breast cancer cells. (A) Scratch wound healing assay of MDA-MB-231 cells. 5 × 105 MDA-MB-231 cells (Control and Restin overexpression) were seeded onto 6-well plates. The 6-well plates were scratched (time 0) and 20 random fields of view per well were photographed after 12 h of scratch. Representative pictures from 3 independent experiments were shown. The motility of MDA-MB-231 and MDA-MB-451 cells was evaluated by transwell migration (B) and Matrigel invasion assays (C) as described in the Materials and Methods. Migration was quantitated by counting 10 fields at a magnification of × 400. Data were presented as the average number of migrated cells from three independent experiments. * p < 0.05 vs Control group. (D) Adhesion capacity of MDA-MB-231 and MDA-MB-451 cells was measured by cell attachment assay. MDA-MB-231 and MDA-MB-451 cells (Control and Restin overexpression) were seeded onto 96-well plates pretreated with fibronectin for 30 min at a density of 2 × 103 cells/well in triplicate. Cells were allowed to adhere for 2 h and adherent cells were stained with crystal violet and quantified using the absorbance as a measurement at 550 nm. Values were expressed as means ± S.M. of at least three independent experiments. * p < 0.05 vs Control.