Figure 6From: The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cellsResults of RNA expression profiling reveal cell type dependent target genes of HOTAIR. (a) Venn diagram for differentially expressed genes in VM-CUB1_HOTAIR 20 and 5637_HOTAIR 4 cells when compared to the respective vector controls. Three independent RNA preparations of each clone were hybridized to Affymetrix PrimeView 3′IVT arrays. Differentially expressed genes with a significance of p < 0.01 (Benjamini-Hochberg adjusted) were selected. (b) Genes with at least two-fold overexpression in VM-CUB1 and 5637 (p < 0.01) were subjected to GO analysis using DAVID analysis software. GO biological process terms significantly enriched at p < 0.05 after adjustment were considered; the list was edited for multiple similar terms. (c) Changes in HOX gene expression pattern in stably transfected VM-CUB1 and 5637 cells (p < 0.01) displayed as a heatmap. Downregulated genes are indicated by light grey boxes, upregulated genes by dark grey boxes. HOX genes not significantly changed are shown in white.Back to article page