Fig. 2

Imetelstat significantly increases radiotherapy efficiency in vivo. a Experimental design: mice were xenografted (D0) and intra-peritoneal injections were started three days later (D3) either with Imetelstat (IMT, 30 mg/Kg three times a week) or by an equivalent volume of PBS, for four weeks. Two weeks after the treatment was began (D13) mice were or were not concomitantly treated by radiotherapy (RT, 2Gy/day, five days), and imaged by μMRI at day 26 (D26). Injections were stopped at the end of the fourth week and mice were monitored until they developed debilitating disease (date used for the OS). b Kaplan Meier representation of OS as a function of days post-xenograft, for mice treated by PBS (black solid line), Imetelstat (green solid line) or a combination of RT with PBS (dashed black line) or with Imetelstat (dashed green line). LogRank calculated p-values are shown for all treatments (on the left) and for IMT/RT versus PBS/RT (on the right). c Tumor volume determined by μMRI at day 26 is shown for each treatment group. p-values were determined comparing each condition to the PBS condition, and between the PBS/RT versus the IMT/RT (p < 0.05, **p < 0.01, ***p < 0.001, Wilcoxon test). d Display of semi-automatic GBM segmentation on T2-weighted MR images: axial plane (left), sagittal plane (middle), coronal plane (right). A 3D reconstruction (right) showing the localization and the size of GBM (yellow) within the mouse brain (turquoise)