Fig. 3From: Convergence of eicosanoid and integrin biology: 12-lipoxygenase seeks a partnerLC-MS analysis of 12-LOX activity. Following the stimulation with mAb β4 over a time course, each membrane fraction (100,000 × g pellet) was harvested and incubated in DMEM at 37 °C with 10 μM [14C]-AA for 15 min. Cell lipids were extracted and analyzed as described in Materials and Methods. The data were analyzed by LC-MS in triplicate and error bars represent SEMBack to article page