Fig. 5

The α6β4 integrin and 12-LOX activity function in EGF-induced chemotaxis. (a) Migration assay: A431 cells were pretreated with 3E1 antibody for 20 min, then plated on laminin-coated invasion plates either with or without EGF (1 ng/ml) for 3 h in the presence or absence of pharmacological inhibitors as described in Methods. Data represent the mean number of transmigrated cells/microscopic field (± SE). The experiments were repeated three times in triplicate. a, P < 0.01 when compared to no-EGF treated group; b, c, d, P < 0.01 when compared to EGF treated control group. (b) Alternate migration assay: Comparison of chemotaxis of A431 cells toward EGF when stimulated with either mAb 3E1 or laminin in the presence or absence of the 12-LOX specific inhibitor BMD122. (10× magnification) (c) Controls for (b). (d) Absorbance measurements of dye retained by transmigrated cells