Fig. 1

Immunostaining of fibronectin and laminin in LNCaP cells after ECM depletion. LNCaP cells were treated with collagenase, hyaluronidase or a combination of both enzymes before incubation with mouse anti-fibronectin (Anti-Fn) or mouse anti-laminin (Anti-Ln) antibodies, followed by goat anti-mouse AlexaFluor 488-conjugated secondary antibody. Depletion of fibronectin after treatment with collagenase or a combination of both enzymes, but not with hyaluronidase alone was clearly observed. No observable differences could be seen for laminin. Experiments including secondary antibody alone were used as negative controls. Data shown are representative of all three cell lines tested (LNCaP, 9L and MCF-7). Experiments were repeated twice and individual conditions were repeated in triplicates