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Fig. 2 | Molecular Cancer

Fig. 2

From: Modulation of extracellular matrix in cancer is associated with enhanced tumor cell targeting by bacteriophage vectors

Fig. 2

Hyaluronic acid ELISA and Collagen Sirius red assay of cell culture media after collagenase and hyaluronidase treatments of 2D cell monolayers and MCTS of MCF-7, LNCaP and 9L. Cell monolayers or MCTS were treated with hyaluronidase or collagenase, or combination of both enzymes before the culture supernatants were analysed for hyaluronic acid (hyaluronan, HA) or collagen (type I). a Hyaluronic acid ELISA was based on adding biotinylated HA binding proteins to a plate coated with umbilical cord HA and subsequent treatment with peroxidase-conjugated anti-biotin antibodies. The results, show release of HA in to the culture medium primarily after treatment with hyaluronidase or a combination of both enzymes. b Sirius Red assay (chondrex) results showing release of collagen in to the culture medium primarily after treatment with collagenase or a combination of both enzymes. Experiments were repeated twice and individual conditions were repeated in triplicates. Data are presented as mean ± s.e.m

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Molecular Cancer

ISSN: 1476-4598