Skip to main content

Advertisement

Fig. 3 | Molecular Cancer

Fig. 3

From: COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer

Fig. 3

MS identified Nucleolin, GRP-78, α-Enolase and Annexin A2 display O-GalNAc glycosylation. a left: Tn antigen is expressed on Nucleolin, GRP-78, α-Enolase and Annexin A2. Immunoprecipitation and western blot analysis were performed using VVL lectin for Tn antigen precipitation and Nucleolin, GRP-78, α-Enolase and Annexin A2 antibodies for detection of specific protein signals. a right: Protein expression levels of the identified proteins in total cell lysates of COSMC knockdown as well as control cells remained unchanged, except for Annexin 2, which showed a decrease in Panc-1 COSMC knockdown cells. b Vice versa, immunoprecipitation using specific antibodies and detection with VVL lectin was performed as well. c Immunocytochemistry of Panc-1 COSMC knockdown cells and control cells. Left: Aberrant O-glycans, including Tn antigen, were detected using VVL-FITC conjugated lectin (green). Middle left: Nucleolin was detected (red) using an anti-Nucleolin antibody (ab13541). Middle right: Nuclei were stained with DAPI (blue). Right: Overlay of Tn antigen and Nucleolin immunocytochemistry. d Nucleolin, GRP-78, Enolase and Annexin detection in the cell membrane protein fraction. Her2 antibody was used as marker for membrane fraction and GAPDH antibody was used as marker for cytoplasmic proteins

Back to article page