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Fig. 4 | Molecular Cancer

Fig. 4

From: 3-BrPA eliminates human bladder cancer cells with highly oncogenic signatures via engagement of specific death programs and perturbation of multiple signaling and metabolic determinants

Fig. 4

3-BrPA induces severe dysregulation of multiple signaling pathways in bladder cancer cells. (a-d) Representative (three independent experiments) Western blotting profiles of whole-cell protein extracts obtained from RT4, T24 and T24-X cells, grown at ~60 % confluency and exposed to the indicated doses of 3-BrPA for 24 h. The proteins examined were p-Akt-Thr308, p-Akt-Ser473, Akt (a), p-FoxO1-Thr24/p-FoxO3a-Thr32, FoxO1, p-GSK-3α/β-Ser21/9, p-GSK-3β-Ser9, GSK-3α, GSK-3β (b), p-mTOR-Ser2448, mTOR, p-S6-Ser235/236, S6, p-AMPKα1-Ser485/p-AMPKα2-Ser491, p-AMPKα-Thr172, AMPKα (c), p-p44/42 MAPK-Thr202/Tyr204, p44/42 MAPK, p-p38 MAPK-Thr180/Tyr182, p38 MAPK, p-SAPK/JNK-Thr183/Tyr185 and SAPK/JNK (d), while Actin was used as molecule of reference. Brackets and asterisks denote the high MW forms of Akt (a), GSK-3β (b), AMPKα (c) and p44/42 MAPK (d) kinases

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