Fig. 4

3-BrPA induces severe dysregulation of multiple signaling pathways in bladder cancer cells. (a-d) Representative (three independent experiments) Western blotting profiles of whole-cell protein extracts obtained from RT4, T24 and T24-X cells, grown at ~60 % confluency and exposed to the indicated doses of 3-BrPA for 24 h. The proteins examined were p-Akt-Thr³⁰⁸, p-Akt-Ser⁴⁷³, Akt (a), p-FoxO1-Thr²⁴/p-FoxO3a-Thr³², FoxO1, p-GSK-3α/β-Ser^21/9, p-GSK-3β-Ser⁹, GSK-3α, GSK-3β (b), p-mTOR-Ser²⁴⁴⁸, mTOR, p-S6-Ser^235/236, S6, p-AMPKα1-Ser⁴⁸⁵/p-AMPKα2-Ser⁴⁹¹, p-AMPKα-Thr¹⁷², AMPKα (c), p-p44/42 MAPK-Thr²⁰²/Tyr²⁰⁴, p44/42 MAPK, p-p38 MAPK-Thr¹⁸⁰/Tyr¹⁸², p38 MAPK, p-SAPK/JNK-Thr¹⁸³/Tyr¹⁸⁵ and SAPK/JNK (d), while Actin was used as molecule of reference. Brackets and asterisks denote the high MW forms of Akt (a), GSK-3β (b), AMPKα (c) and p44/42 MAPK (d) kinases