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Fig. 6 | Molecular Cancer

Fig. 6

From: 3-BrPA eliminates human bladder cancer cells with highly oncogenic signatures via engagement of specific death programs and perturbation of multiple signaling and metabolic determinants

Fig. 6

B-RafV600E and K-RasG13D oncogenic proteins are not associated with 3-BrPA-induced or glucose deprivation-driven death of bladder cancer cells. (a) DNA sequencing chromatograms (three independent experiments) of B-Raf (left panel) and K-Ras (right panel) genomic PCR products amplified from RT4, T24 and T24-X cells, using gene-specific primers flanking the V600 and G12/G13 cognate codons, respectively (also, see Additional file 4: Figure S4, Additional file 9: Table S1 and Additional file 10: Table S2). F/R: forward/reverse -DNA sequence- reading direction. (b-f) MTT cytotoxicity assays of RT4 (b), T24 (c), T24-X (d), RT112 (e) and TCCSUP (f) cells, grown at ~60 % confluency in glucose-free 1X DMEM complete medium (containing 10 % FBS, 2 mM L-glutamine, 1 mM sodium pyruvate, 50 mM sodium bicarbonate, 1X non-essential amino acids, 100 u/ml penicillin and 100 μg/ml streptomycin), and harvested for further processing at the indicated time points (days) (also, see Additional file 10: Table S2). (b-f) Data are reported as mean ± standard deviation of triplicates of three independent experiments. *P < 0.001

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