Fig. 6

EREG expression correlates with MMP-1 levels in human DCIS lesions. a qRT-PCR analysis of MMP-1 gene expression in MCF10A and MCF10DCIS cells. Expression levels of MMP-1 are normalized to CYBP. b Immunoblot analysis of MMP-1 in conditioned media obtained from MCF10A and MCF10DCIS cells. Loading was assessed by Coomassie staining (Additional file 1: Figure S1C). c qRT-PCR analysis of MMP-1 gene expression in MCF10DCIS cells expressing either NT or EREG shRNA constructs. Expression levels of MMP-1 are normalized to CYBP. d Immunohistochemistry of normal breast tissue and DCIS stained with an anti-EREG antibody. Scale bars represent 50 μm. e Normal and DCIS human samples were stained with an anti-MMP-1 antibody. Representative images of normal and varying levels of MMP-1 staining in DCIS lesions are shown. f Quantification of the percent of samples staining positive for EREG. g Quantification of the percent of samples staining positive for MMP-1. Scale bars represent 50 μm. *p < 0.05. ***p < 0.0001