Fig. 1

SgK223 in pancreatic cancer. a-b Site-selective tyrosine phosphorylation of SgK223 across a PDAC cell line panel, determined through mass spectrometry (Humphrey et al. manuscript in preparation). The heatmaps indicate relative tyrosine phosphorylation of SgK223 at (a) Y159; and (b) Y411. c SgK223 protein expression across a panel of PDAC cell lines, determined by Western blotting. SgK223 protein expression in the non-transformed pancreatic cell line, HPDE, and an overexpression cell line model utilising HPDE cells are also shown in the same immunoblot. d SgK223 is overexpressed in PDAC compared to normal pancreas. The box plot indicates SgK223 mRNA expression in PDAC versus normal pancreatic tissue from the same patient (6 cancer specimens and 6 normal controls were analysed). Boxes represent the interquartile range (IQR) between the 75th percentile and the 25th percentile (Q3 - Q1), and the 50th percentile (median; Q2) is represented as the line within the boxes. Error bars were calculated as Q1 - 1.5 (IQR) and Q3 + 1.5 (IQR). ***indicates significance with a P-value < 0.0005