Fig. 1

Phenotypic screening in primary NSCLC tumour cells. a Images of cells derived from NSCLC primary tumour #1 cultured in three different conditions. b Effects of the scFv-Fc antibody panel upon NSCLC tumour #1 cell growth in three culture conditions, measured by Cell-Titre Glo (CTG) luminescence signal. Each antibody was individually dosed (without normalising concentrations) and cells were grown in 96-well plates. Positive (anti-IGF1R) and negative control antibodies were dosed in multiple replicates on each plate to establish consistency between plates. Each data point indicates a single well. Black horizontal bars indicate the average value for a sample class. c Scatter plot comparing the effects of scFv-Fc antibodies on NSCLC tumour #1 cell growth grown in spheroids and in standard monolayer cultures. Each data point indicates a single antibody (or replicate of the controls). The dashed box indicates a group of antibodies that strongly inhibited growth of cell monolayers but not spheroids. The solid-line box indicates a group of antibodies with a weak inhibitory effect in both spheroids and monolayers. The orange-coloured datapoint represents an antibody that was later shown to bind CDCP1 (αCDCP1-Ab3 in Fig. 2)