Fig. 8

a Expression of Oct4, Lin28, Sox2, CA125, Ki67 and Bcl-2 in mouse tumour xenografts generated by vector control, Oct4A KD1 and Oct4A KD2 cells. Representative immunohistochemistry staining images of debulked mouse xenografts for the expression of Oct4, Lin28 and Sox2. b Representative immunohistochemistry staining images of debulked mouse xenografts for the expression of CA125, Ki67 and Bcl-2. All images are set at 200x magnification and scale bar represents 50 μM. Quantification of antibody staining was determined by using Image J software recognizing DAB intensity. Variations in staining were determined by subtracting the negative control DAB reading from the protein of interest DAB reading for each xenograft. Data is presented as the mean ± SEM of staining intensity (n = 4/group). Significant variations between Oct4A KD groups and vector control were determined by student’s t-test *p < 0.05; **p < 0.01