Fig. 1

PD-L1 is associated with mesenchymal status of normal and malignant breast. a Normal and breast cancer established cell lines grouped into luminal-like, basal/mesenchymal-like breast cancer cells and others including normal breast cell lines. b Representative flow cytometry dot plots showing the phenotype of PD-L1 positive segregated normal human breast cells as measured by Ep-CAM/CD49f, CD44/CD24 and CD90/CD31 expression and as analyzed by multi-parametric flow cytometry following serial gating to remove debris, dead and hematopoietic cells. c Representative flow cytometry histogram showing the level of PD-L1 expression in primary cultured epithelial (luminal and myoepithelial) and mesenchymal cells